Electrokinetic Thermal Cycler and Reactor
LSU Reference: 0509
Status:
- Under Patent Prosecution
Description:
A powerful, miniaturized “lab-on-a-chip” permits faster and more accurate DNA analysis
Applications:
- “Real-time” user-controlled polymerase chain reactions
- Rapid pathogen identification
- Forensics
Advantages:
- Highly automated with “real-time” monitoring & user-control capabilities
- On-board, label-free reaction product detection
- Inexpensive
Abstract: An automated, electrokinetically-driven continuous-flow microfluidic device is capable of specimen heating and cooling at previously unattainable rates, vastly reducing polymerase chain reaction (PCR) reaction times. The device’s closed-loop architecture permits the specimen to be thermally cycled an arbitrary number of times, enabling time-efficient termination or the addition of reagents at the operator’s discretion. Integrated electroimpedance monitoring circuits are capable of real-time, label-free monitoring of reaction products such as PCR amplicons. The device may be inexpensively mass-produced, making it potentially disposable, reducing the risks of sample contamination
This page was last updated Friday, November 19, 2010
